human il 2 (R&D Systems)
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Human Il 2, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 915 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+recombinant+il+2/Recombinant+Human+IL-2+Protein/pmc13592517-547-23-27
Average 96 stars, based on 915 article reviews
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Recombinant:Article Title: A human neuron alzheimer’s disease model reveals barriers to senolytic translatability Article Snippet: NK92 cells (ATCC, CRL-2407) were cultured following the vendor’s instructions. .. Complete growth media was prepared using alpha MEM media (Gibco, 12561072) supplemented with 12.5% non-heat-inactivated fetal bovine serum (VWR, 45000-73), 12.5% heat-inactivated horse serum (ThermoFisher, 26050088), 0.02 mM folic acid (Sigma, F8758-5G), 0.2 mM myo-Inositol (Sigma, I7508-50G), 0.1 mM beta-mercaptoethanol (ThermoFisher, 21985023), 100 U/mL Penicillin-Streptomycin solution (ThermoFisher, 15070063), and 100 U/mL Article Title: Occlusion of TCR binding to HLA-A*11:01 by a non-pathogenic human alloantibody. Article Snippet: To expand antigenspecific T cell lines in fresh PBMC, 1 × 106 PBMCs per well were cultivated in the RPMI-1640 media supplemented with 5% heat-inactivated AB human serum (Gibco), 100 units/ ml penicillin, 100 mg/ml streptomycin, 2 mM L-glutamine and 40 μM of 2-Mercaptoethanol in 12 well plates in the presence of 5 μg/ml various HLA-A*11:01-specific peptides (Supplementary Table 1) and maintained at 37 °C with 5% CO2. .. On day 2, 50 U/ml Article Title: Antigen stimulation drives clonal expansion of latent CD4 + T cells using a full-length HIV latency reporter Article Snippet: To generate autologous central memory T cell populations, naïve CD4 + T cells from the same donor were activated with anti-human CD3e/CD28 antibody coated Dynabeads (1:1 bead:cell ratio, Life Technologies; cat #11131D) and expanded in the presence of rhTGFb (10ng/ml), anti-human IL-4 (1μg/ml), anti-human IL-12 antibody (2μg/ml) and anti-human IL-23 antibody (2μg/ml) in Immunocult-XF expansion media, as previously described ( ). .. After 48 hours, beads were magnetically removed using an EasySep magnet (Stemcell Technologies; cat# 18000) and T cells were cultured for another 5 days in Immunocult-XF media supplemented with 50 IU/mL Article Title: A human neuron alzheimer's disease model reveals barriers to senolytic translatability. Article Snippet: NK92 cells (ATCC, CRL-2407) were cultured following the vendor’s instructions. .. Complete growth media was prepared using alpha MEM media (Gibco, 12561072) supplemented with 12.5% non-heat-inactivated fetal bovine serum (VWR, 45000-73), 12.5% heat-inactivated horse serum (ThermoFisher, 26050088), 0.02 mM folic acid (Sigma, F8758-5G), 0.2 mM myo-Inositol (Sigma, I7508-50G), 0.1 mM beta-mercaptoethanol (ThermoFisher, 21985023), 100 U/mL Penicillin-Streptomycin solution (ThermoFisher, 15070063), and 100 U/mL Article Title: A Human Neuron Alzheimer’s Disease Model Reveals Barriers to Senolytic Translatability Article Snippet: NK92 cells (ATCC, CRL-2407) were cultured following the vendor’s instructions. .. Complete growth media was prepared using alpha MEM media (Gibco, 12561072) supplemented with 12.5% non-heat-inactivated fetal bovine serum (VWR, 45000-73), 12.5% heat-inactivated horse serum (ThermoFisher, 26050088), 0.02 mM folic acid (Sigma, F8758-5G), 0.2 mM myo-Inositol (Sigma, I7508-50G), 0.1 mM beta-mercaptoethanol (ThermoFisher, 21985023), 100 U/mL Penicillin-Streptomycin solution (ThermoFisher, 15070063), and 100 U/mL Article Title: Occlusion of TCR binding to HLA-A*11:01 by a non-pathogenic human alloantibody Article Snippet: To expand antigen-specific T cell lines in fresh PBMC, 1 × 10 6 PBMCs per well were cultivated in the RPMI-1640 media supplemented with 5% heat-inactivated AB human serum (Gibco), 100 units/ml penicillin, 100 mg/ml streptomycin, 2 mM L-glutamine and 40 μM of 2-Mercaptoethanol in 12 well plates in the presence of 5 μg/ml various HLA-A*11:01-specific peptides (Supplementary Table 1) and maintained at 37 °C with 5% CO 2. .. On day 2, 50 U/ml Article Title: Evaluating the Influence of Different Serum‐Free Culture Conditions on the Production and Function of Natural Killer Cell‐Derived Extracellular Vesicles Article Snippet: .. NK‐92 cells (ATCC CRL‐2407) were maintained in Advanced RPMI 1640 (ThermoFisher) supplemented with 20% heat inactivated FBS (Merck), 100 U/mL penicillin‐streptomycin (ThermoFisher), 1× GlutaMAX supplement (ThermoFisher), 50 nM 2‐mercaptoethanol (ThermoFisher), and Article Title: Artificial cell-derived vesicles by extrusion, a novel docetaxel drug delivery system for lung cancer. Article Snippet: Lung cancer (LC) has the highest mortality rate worldwide and novel therapies are being sought.. Among those are cell-product-based therapies such as extracellular vesicles (EVs).. Recently, it has been discovered that artificial cell-derived vesicle by extrusion (EXT) could be a potential tool to lower barriers to clinical translation. Cell Culture:Article Title: Antigen stimulation drives clonal expansion of latent CD4 + T cells using a full-length HIV latency reporter Article Snippet: To generate autologous central memory T cell populations, naïve CD4 + T cells from the same donor were activated with anti-human CD3e/CD28 antibody coated Dynabeads (1:1 bead:cell ratio, Life Technologies; cat #11131D) and expanded in the presence of rhTGFb (10ng/ml), anti-human IL-4 (1μg/ml), anti-human IL-12 antibody (2μg/ml) and anti-human IL-23 antibody (2μg/ml) in Immunocult-XF expansion media, as previously described ( ). .. After 48 hours, beads were magnetically removed using an EasySep magnet (Stemcell Technologies; cat# 18000) and T cells were cultured for another 5 days in Immunocult-XF media supplemented with 50 IU/mL Article Title: Artificial cell-derived vesicles by extrusion, a novel docetaxel drug delivery system for lung cancer. Article Snippet: Lung cancer (LC) has the highest mortality rate worldwide and novel therapies are being sought.. Among those are cell-product-based therapies such as extracellular vesicles (EVs).. Recently, it has been discovered that artificial cell-derived vesicle by extrusion (EXT) could be a potential tool to lower barriers to clinical translation. |
