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human il 2  (R&D Systems)


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    Structured Review

    R&D Systems human il 2
    Human Il 2, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 915 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+recombinant+il+2/Recombinant+Human+IL-2+Protein/pmc13592517-547-23-27
    Average 96 stars, based on 915 article reviews
    human il 2 - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Recombinant:

    Article Title: A human neuron alzheimer’s disease model reveals barriers to senolytic translatability
    Article Snippet: NK92 cells (ATCC, CRL-2407) were cultured following the vendor’s instructions. .. Complete growth media was prepared using alpha MEM media (Gibco, 12561072) supplemented with 12.5% non-heat-inactivated fetal bovine serum (VWR, 45000-73), 12.5% heat-inactivated horse serum (ThermoFisher, 26050088), 0.02 mM folic acid (Sigma, F8758-5G), 0.2 mM myo-Inositol (Sigma, I7508-50G), 0.1 mM beta-mercaptoethanol (ThermoFisher, 21985023), 100 U/mL Penicillin-Streptomycin solution (ThermoFisher, 15070063), and 100 U/mL human recombinant IL-2 (R&D Systems, 202-IL). .. The prepared media was equilibrated in T25 flasks (Corning) in humidified incubator at 37 °C with 5% CO2 and 20% O2.

    Article Title: Occlusion of TCR binding to HLA-A*11:01 by a non-pathogenic human alloantibody.
    Article Snippet: To expand antigenspecific T cell lines in fresh PBMC, 1 × 106 PBMCs per well were cultivated in the RPMI-1640 media supplemented with 5% heat-inactivated AB human serum (Gibco), 100 units/ ml penicillin, 100 mg/ml streptomycin, 2 mM L-glutamine and 40 μM of 2-Mercaptoethanol in 12 well plates in the presence of 5 μg/ml various HLA-A*11:01-specific peptides (Supplementary Table 1) and maintained at 37 °C with 5% CO2. .. On day 2, 50 U/ml human recombinant IL-2 (R&D System) was added and from day 5 half medium change and supplementation of IL-2 was performed every 2 to 3 days (Fig. 2a). ..

    Article Title: Antigen stimulation drives clonal expansion of latent CD4 + T cells using a full-length HIV latency reporter
    Article Snippet: To generate autologous central memory T cell populations, naïve CD4 + T cells from the same donor were activated with anti-human CD3e/CD28 antibody coated Dynabeads (1:1 bead:cell ratio, Life Technologies; cat #11131D) and expanded in the presence of rhTGFb (10ng/ml), anti-human IL-4 (1μg/ml), anti-human IL-12 antibody (2μg/ml) and anti-human IL-23 antibody (2μg/ml) in Immunocult-XF expansion media, as previously described ( ). .. After 48 hours, beads were magnetically removed using an EasySep magnet (Stemcell Technologies; cat# 18000) and T cells were cultured for another 5 days in Immunocult-XF media supplemented with 50 IU/mL human recombinant IL-2 (R&D systems; cat #200-02), keeping cell densities at 2 x 10 5 cells/mL. ..

    Article Title: A human neuron alzheimer's disease model reveals barriers to senolytic translatability.
    Article Snippet: NK92 cells (ATCC, CRL-2407) were cultured following the vendor’s instructions. .. Complete growth media was prepared using alpha MEM media (Gibco, 12561072) supplemented with 12.5% non-heat-inactivated fetal bovine serum (VWR, 45000-73), 12.5% heat-inactivated horse serum (ThermoFisher, 26050088), 0.02 mM folic acid (Sigma, F8758-5G), 0.2 mM myo-Inositol (Sigma, I7508-50G), 0.1 mM beta-mercaptoethanol (ThermoFisher, 21985023), 100 U/mL Penicillin-Streptomycin solution (ThermoFisher, 15070063), and 100 U/mL human recombinant IL-2 (R&D Systems, 202-IL). .. The prepared media was equilibrated in T25 flasks (Corning) in humidified incubator at 37 °C with 5% CO2 and 20% O2.

    Article Title: A Human Neuron Alzheimer’s Disease Model Reveals Barriers to Senolytic Translatability
    Article Snippet: NK92 cells (ATCC, CRL-2407) were cultured following the vendor’s instructions. .. Complete growth media was prepared using alpha MEM media (Gibco, 12561072) supplemented with 12.5% non-heat-inactivated fetal bovine serum (VWR, 45000-73), 12.5% heat-inactivated horse serum (ThermoFisher, 26050088), 0.02 mM folic acid (Sigma, F8758-5G), 0.2 mM myo-Inositol (Sigma, I7508-50G), 0.1 mM beta-mercaptoethanol (ThermoFisher, 21985023), 100 U/mL Penicillin-Streptomycin solution (ThermoFisher, 15070063), and 100 U/mL human recombinant IL-2 (R&D Systems, 202-IL). .. The prepared media was equilibrated in T25 flasks (Corning) in humidified incubator at 37 °C with 5% CO2 and 20% O2.

    Article Title: Occlusion of TCR binding to HLA-A*11:01 by a non-pathogenic human alloantibody
    Article Snippet: To expand antigen-specific T cell lines in fresh PBMC, 1 × 10 6 PBMCs per well were cultivated in the RPMI-1640 media supplemented with 5% heat-inactivated AB human serum (Gibco), 100 units/ml penicillin, 100 mg/ml streptomycin, 2 mM L-glutamine and 40 μM of 2-Mercaptoethanol in 12 well plates in the presence of 5 μg/ml various HLA-A*11:01-specific peptides (Supplementary Table 1) and maintained at 37 °C with 5% CO 2. .. On day 2, 50 U/ml human recombinant IL-2 (R&D System) was added and from day 5 half medium change and supplementation of IL-2 was performed every 2 to 3 days (Fig. a). ..

    Article Title: Evaluating the Influence of Different Serum‐Free Culture Conditions on the Production and Function of Natural Killer Cell‐Derived Extracellular Vesicles
    Article Snippet: .. NK‐92 cells (ATCC CRL‐2407) were maintained in Advanced RPMI 1640 (ThermoFisher) supplemented with 20% heat inactivated FBS (Merck), 100 U/mL penicillin‐streptomycin (ThermoFisher), 1× GlutaMAX supplement (ThermoFisher), 50 nM 2‐mercaptoethanol (ThermoFisher), and human recombinant IL‐2 (R&D Systems) at 50 ng/mL. ..

    Article Title: Artificial cell-derived vesicles by extrusion, a novel docetaxel drug delivery system for lung cancer.
    Article Snippet: Lung cancer (LC) has the highest mortality rate worldwide and novel therapies are being sought.. Among those are cell-product-based therapies such as extracellular vesicles (EVs).. Recently, it has been discovered that artificial cell-derived vesicle by extrusion (EXT) could be a potential tool to lower barriers to clinical translation.

    Cell Culture:

    Article Title: Antigen stimulation drives clonal expansion of latent CD4 + T cells using a full-length HIV latency reporter
    Article Snippet: To generate autologous central memory T cell populations, naïve CD4 + T cells from the same donor were activated with anti-human CD3e/CD28 antibody coated Dynabeads (1:1 bead:cell ratio, Life Technologies; cat #11131D) and expanded in the presence of rhTGFb (10ng/ml), anti-human IL-4 (1μg/ml), anti-human IL-12 antibody (2μg/ml) and anti-human IL-23 antibody (2μg/ml) in Immunocult-XF expansion media, as previously described ( ). .. After 48 hours, beads were magnetically removed using an EasySep magnet (Stemcell Technologies; cat# 18000) and T cells were cultured for another 5 days in Immunocult-XF media supplemented with 50 IU/mL human recombinant IL-2 (R&D systems; cat #200-02), keeping cell densities at 2 x 10 5 cells/mL. ..

    Article Title: Artificial cell-derived vesicles by extrusion, a novel docetaxel drug delivery system for lung cancer.
    Article Snippet: Lung cancer (LC) has the highest mortality rate worldwide and novel therapies are being sought.. Among those are cell-product-based therapies such as extracellular vesicles (EVs).. Recently, it has been discovered that artificial cell-derived vesicle by extrusion (EXT) could be a potential tool to lower barriers to clinical translation.



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    Assessment of auxCAR‐T cell performance in Jurkat T cells. (A,B) Representative Flow cytometry (A) and Statistical quantification (B) of early T cell activation as reflected by upregulated CD69 expression on the cell surface. Jurkat T cells transduced with stCAR (left) and auxCAR (right) were co‐cultured with hCD19 + Raji cells or hCD19 − K562 cells, with or without auxin. n = 3 independent biological replicates (mean ± s.d.). The p ‐values were calculated using one‐way analysis of variance (ANOVA). (C,D) Statistical quantification of NFAT‐GFP reporter activity in Jurkat T cells expressing stCAR and auxCAR co‐cultured with tumor cells with hCD19 + Raji cells (C) or A431‐CD19 (D) with the indicated auxin concentration. n = 3 independent technical replicates (mean ± s.d.). The p ‐values were calculated using one‐way analysis of variance (ANOVA). (E) Auxin‐dose‐dependent responses of stCAR T and auxCAR‐T cells examined <t>by</t> <t>IL‐2</t> production in Jurkat T cells. (F) Architecture of the auxCAR HER2. Upper: cartoon depicting the different components. Bottom: schematic of the R‐ and S‐chains encoded in a single lentiviral vector, each separated by Venus or mScarlet. (G,H) Statistical quantification of NFAT‐GFP reporter activity (G) and IL‐2 production (H) in Jurkat T cells expressing stCAR HER2 or auxCAR HER2 co‐cultured with SK‐OV‐3 tumor cells in the presence of the indicated auxin concentration. n = 3 independent technical replicates (mean ± s.d.). (I) Statistical quantification of NFAT‐GFP reporter activity at the indicated time points following auxin removal in Jurkat T cells expressing auxCAR and co‐cultured with SK‐OV‐3 tumor cells. The auxCAR T cells were induced with 1 µM auxin.
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    Assessment of auxCAR‐T cell performance in Jurkat T cells. (A,B) Representative Flow cytometry (A) and Statistical quantification (B) of early T cell activation as reflected by upregulated CD69 expression on the cell surface. Jurkat T cells transduced with stCAR (left) and auxCAR (right) were co‐cultured with hCD19 + Raji cells or hCD19 − K562 cells, with or without auxin. n = 3 independent biological replicates (mean ± s.d.). The p ‐values were calculated using one‐way analysis of variance (ANOVA). (C,D) Statistical quantification of NFAT‐GFP reporter activity in Jurkat T cells expressing stCAR and auxCAR co‐cultured with tumor cells with hCD19 + Raji cells (C) or A431‐CD19 (D) with the indicated auxin concentration. n = 3 independent technical replicates (mean ± s.d.). The p ‐values were calculated using one‐way analysis of variance (ANOVA). (E) Auxin‐dose‐dependent responses of stCAR T and auxCAR‐T cells examined <t>by</t> <t>IL‐2</t> production in Jurkat T cells. (F) Architecture of the auxCAR HER2. Upper: cartoon depicting the different components. Bottom: schematic of the R‐ and S‐chains encoded in a single lentiviral vector, each separated by Venus or mScarlet. (G,H) Statistical quantification of NFAT‐GFP reporter activity (G) and IL‐2 production (H) in Jurkat T cells expressing stCAR HER2 or auxCAR HER2 co‐cultured with SK‐OV‐3 tumor cells in the presence of the indicated auxin concentration. n = 3 independent technical replicates (mean ± s.d.). (I) Statistical quantification of NFAT‐GFP reporter activity at the indicated time points following auxin removal in Jurkat T cells expressing auxCAR and co‐cultured with SK‐OV‐3 tumor cells. The auxCAR T cells were induced with 1 µM auxin.
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    Image Search Results


    Assessment of auxCAR‐T cell performance in Jurkat T cells. (A,B) Representative Flow cytometry (A) and Statistical quantification (B) of early T cell activation as reflected by upregulated CD69 expression on the cell surface. Jurkat T cells transduced with stCAR (left) and auxCAR (right) were co‐cultured with hCD19 + Raji cells or hCD19 − K562 cells, with or without auxin. n = 3 independent biological replicates (mean ± s.d.). The p ‐values were calculated using one‐way analysis of variance (ANOVA). (C,D) Statistical quantification of NFAT‐GFP reporter activity in Jurkat T cells expressing stCAR and auxCAR co‐cultured with tumor cells with hCD19 + Raji cells (C) or A431‐CD19 (D) with the indicated auxin concentration. n = 3 independent technical replicates (mean ± s.d.). The p ‐values were calculated using one‐way analysis of variance (ANOVA). (E) Auxin‐dose‐dependent responses of stCAR T and auxCAR‐T cells examined by IL‐2 production in Jurkat T cells. (F) Architecture of the auxCAR HER2. Upper: cartoon depicting the different components. Bottom: schematic of the R‐ and S‐chains encoded in a single lentiviral vector, each separated by Venus or mScarlet. (G,H) Statistical quantification of NFAT‐GFP reporter activity (G) and IL‐2 production (H) in Jurkat T cells expressing stCAR HER2 or auxCAR HER2 co‐cultured with SK‐OV‐3 tumor cells in the presence of the indicated auxin concentration. n = 3 independent technical replicates (mean ± s.d.). (I) Statistical quantification of NFAT‐GFP reporter activity at the indicated time points following auxin removal in Jurkat T cells expressing auxCAR and co‐cultured with SK‐OV‐3 tumor cells. The auxCAR T cells were induced with 1 µM auxin.

    Journal: Advanced Science

    Article Title: Repurposing a Small Molecule Plant Hormone as a Tunable ON‐Switch for CAR‐T Cell Immunotherapy

    doi: 10.1002/advs.77020

    Figure Lengend Snippet: Assessment of auxCAR‐T cell performance in Jurkat T cells. (A,B) Representative Flow cytometry (A) and Statistical quantification (B) of early T cell activation as reflected by upregulated CD69 expression on the cell surface. Jurkat T cells transduced with stCAR (left) and auxCAR (right) were co‐cultured with hCD19 + Raji cells or hCD19 − K562 cells, with or without auxin. n = 3 independent biological replicates (mean ± s.d.). The p ‐values were calculated using one‐way analysis of variance (ANOVA). (C,D) Statistical quantification of NFAT‐GFP reporter activity in Jurkat T cells expressing stCAR and auxCAR co‐cultured with tumor cells with hCD19 + Raji cells (C) or A431‐CD19 (D) with the indicated auxin concentration. n = 3 independent technical replicates (mean ± s.d.). The p ‐values were calculated using one‐way analysis of variance (ANOVA). (E) Auxin‐dose‐dependent responses of stCAR T and auxCAR‐T cells examined by IL‐2 production in Jurkat T cells. (F) Architecture of the auxCAR HER2. Upper: cartoon depicting the different components. Bottom: schematic of the R‐ and S‐chains encoded in a single lentiviral vector, each separated by Venus or mScarlet. (G,H) Statistical quantification of NFAT‐GFP reporter activity (G) and IL‐2 production (H) in Jurkat T cells expressing stCAR HER2 or auxCAR HER2 co‐cultured with SK‐OV‐3 tumor cells in the presence of the indicated auxin concentration. n = 3 independent technical replicates (mean ± s.d.). (I) Statistical quantification of NFAT‐GFP reporter activity at the indicated time points following auxin removal in Jurkat T cells expressing auxCAR and co‐cultured with SK‐OV‐3 tumor cells. The auxCAR T cells were induced with 1 µM auxin.

    Article Snippet: Recombinant human IL‐2 (Cat# RP01039, ABclonal) was added at a final concentration of 100 Unit/mL −1 to expand T cells.

    Techniques: Flow Cytometry, Activation Assay, Expressing, Transduction, Cell Culture, Activity Assay, Concentration Assay, Plasmid Preparation